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KMID : 0380620150470010020
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 1 p.20 ~ p.26
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Discrimination of Cultivars and Cultivation Origins from the Sepals of Dry Persimmon Using FT-IR Spectroscopy Combined with Multivariate Analysis
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Hur Suel-Hye
Kim Suk-Weon
Min Byung-Whan
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Abstract
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This study aimed to establish a rapid system for discriminating the cultivation origins and cultivars of dry persimmons, using metabolite fingerprinting by Fourier transform infrared (FT-IR) spectroscopy combined with multivariate analysis. Whole-cell extracts from the sepals of four Korean cultivars and two different Chinese dry persimmons were subjected to FT-IR spectroscopy. Principle component analysis (PCA) and partial least squares discriminant analysis (PLSDA) of the FT-IR spectral data successfully discriminated six dry persimmons into two groups depending on their cultivation origins. Principal component loading values showed that the 1750-1420 and 1190-950 cm?1 regions of the FTIR spectra were significantly important for the discrimination of cultivation origins. The accuracy of prediction of the cultivation origins and cultivars by PLS regression was 100% (p<0.01) and 85.9% (p<0.05), respectively. These results clearly show that metabolic fingerprinting of FT-IR spectra can be applied for rapid discrimination of the cultivation origins and cultivars of commercial dry persimmons
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KEYWORD
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dry persimmon, sepal, Fourier transformation infrared (FT-IR) spectroscopy, principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA)
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